The Serbian parliament has voted overwhelmingly to condemn any attempt by Kosovo to become independent, BBC reported Thursday.
Authorities in the province say they will declare independence unilaterally in the coming weeks, as talks with Serbia have made no progress.
The Belgrade parliament's resolution calls for action against those who recognise an independent Kosovo.
After eight hours of debate on Wednesday, Serbian MPs approved the resolution by 220 votes to 14.
It rejects the idea of the EU setting up a mission in Kosovo before the province's status is resolved and says Serbia will "reconsider" diplomatic ties with Western countries that recognise Kosovo's statehood.
Prime Minister Vojislav Kostunica stressed Serbia would not join the EU if the bloc recognised Kosovo's independence.
The opposition leader Cedomir Jovanovic said the resolution was a blow to Serbia's ambitions to become an EU member.
The province is still formally part of Serbia, but has been administered by the United Nations since 1999.